KMID : 0355620080340040419
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Journal of Korean Association of Oral and Maxillofacial Surgeons 2008 Volume.34 No. 4 p.419 ~ p.427
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The effects of ¥â-TCP/rhBMP-2 on bone formation in osteoblast-like cells induced from bone marrow-derived mesenchymal stem cells
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Choi Yong-Soo
Hwang Kyung-Gyun Shim Kwang-Sub Park Chang-Joo Lee Jae-Sun
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Abstract
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The present study aimed to investigate the osteogenic potentials of differentiated osteoblast-like cells (DOCs) induced from bone marrow-derived mesenchymal stem cells (MSCs) on ¥â-tricalcium phosphate (¥â-TCP) with recombinant human bone morphogenetic protein (rhBMP-2) in vitro. Osteoblast differentiation was induced in confluent cultures by adding 100 nM dexamethasone, 10 mM ¥â-glycerophosphate, 50 mM L-ascorbic acid. The Alizarin red S staining and reverse transcriptase-polymerase chain reaction (RT-PCR) were perfomed to examine the mRNA expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN), receptor activator for nuclear factor ¥êB ligand (RANKL), runt-related transcription factor 2 (RUNX2), collagen-¥° (COL-¥°). There were no significant differences in the osteogenic potentials of DOCs induced from MSCs on ¥â-TCP(+/-). According to the incubation period, there were significant increasing of Alizadin red S staining in the induction 3 weeks. The mRNA expression of ALP, RUNX2, and RANKL were higher in DOCs/¥â-TCP(-) than DOCs/¥â-TCP(+). According to rhBMP-2 concentrations, the mRNA expression of BSP was significantly increased in DOCs/¥â-TCP(+) compared to that of DOCs/¥â-TCP(-) on rhBMP 10 ng/ml. Our study presented the ¥â-TCP will have the possibility that calcium phosphate directly affect the osteoblastic differentiation of the bone marrowderived MSCs.
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KEYWORD
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mesenchymalstemcell, rhBMP-2, ¥â-TCP, differentiatedosteoblast-likecell
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